Part:BBa_K4211015:Design
REDMAP-MazF
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene of interest (GOI) is replaced with MazF by BNUZH-China iGEM team 2022 to cause the engineered cells to commit suicide.
Source
It was designed by the BNUZH-China iGEM team 2022 based on the article in Nature Biotechnology: A small and highly sensitive red/far-red optogenetic switch for applications in mammals.
References
[1] Park, J. H., Yamaguchi, Y. & Inouye, M. Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase. FEBS Lett 585, 2526-2532 (2011). https://doi.org:10.1016/j.febslet.2011.07.008
[2] Zhou, Y., Kong, D., Wang, X. et al. A small and highly sensitive red/far-red optogenetic switch for applications in mammals. Nat Biotechnol 40, 262–272 (2022). https://doi.org/10.1038/s41587-021-01036-w
[3] Simanshu, D. K., Yamaguchi, Y., Park, J. H., Inouye, M. & Patel, D. J. Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis. Mol Cell 52, 447-458 (2013). https://doi.org:10.1016/j.molcel.2013.09.006
[4] Stein, T. Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol 56, 845-857 (2005). https://doi.org:10.1111/j.1365-2958.2005.04587.x
[5] Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L. & Condon, C. The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56, 1139-1148 (2005). https://doi.org:10.1111/j.1365-2958.2005.04606.x